Show pageOld revisionsBacklinksBack to top This page is read only. You can view the source, but not change it. Ask your administrator if you think this is wrong. ====== TISSUE FIXATION ====== * Tissue fixation * aim and objectives of fixation * prevent autolysis and putrefaction of sample * cause * coagulation of tissue * to prevent loss of diffusible substances * stabilization of proteins * to fortify or increase strength of tissue specimen * to make favorable condition for stains * tissue fixatives or chemical fixatives * Crosslinking fixatives – aldehydes * formaldehyde or formalin * glutaraldehyde * Precipitating (denaturing ) fixatives – alcohols * Ethyl Alcohol * methanol ( sometimes) * oxidising agents * for histology * potassium dichromate * potassium permanganate * chromic acid * for electron microscopy * osmium tetroxide * mercurials * use * increases staining brightness * but slow permeability * types * mercuric chloride * zenker's fixative * Picrates * bouins fluid * Karnovsky fixative ===== TISSUE FIXATIVES ===== ==== Formaldehyde or formalin ==== ==== bouins fluid ==== ==== Glutaraldehyde ==== ==== Mercuric chloride ==== ==== Potassium Dichromate ==== ==== Ethyl Alcohol ==== en/tissue_fixation.txt Last modified: 2023/07/04 10:06by 127.0.0.1