Microtome

Microtome
- types
  - Hand Microtome
  - Bench Microtome
  - Rocking Microtome
  - Sledge or Sliding Microtome
  - Freezing Microtome
  - Rotary Microtome
  - Cold Microtome or Cryostat
- Basic parts
  - knife
    - material
      - made up of high quality steel , glass or diamond
    - relation to object
      - angle and direction
        
        slant of knife
        
        zero degrees
        
        45 degrees
        
        30 degrees
    - types
      - Profile A
        
        Biconcave
        
        hollow ground on both sides
      - Profile B
        
        Planoconcave
        
        hollow ground on one side
      - Profile C
        
        wedge shaped
        
        used for sectioning
      - Profile D
        
        tool edge
        
        used for hard materials like wood
    - measures
      - temperature of knife
      - cutting rate
      - safety from injury
      - contamination
      - orientation of block
- Actions
  - cutting
  - Sharpening
    - of knife
      - Honing
      - Stroping or polishing

SECTIONAL METHOD

Sectional method